have made a dramatic impact on gene expression profiling
studies. However, this format has not been standardized
until now and is still suffering from low sensitivity
for RNA (or cDNA) and undesirable variation within a
microarray. Therefore, the NSB slide is expected
to provide a gold standard DNA microarray with the
highest sensitivity and reliability for gene expression profiling.
For comparison with one of the most popular competing slides,
gene expression profiling studies were performed using cDNAs reverse-transcripted
out of total RNA without further amplification process.
As shown in the figure below,
NSB slide shows very high sensitivity even
using 1 レg total RNA, while the competitor has many
missing spots as well as lower signal intensity than
the NSB slide. Moreover, the NSB slide maintains a
very low background signal for 1 レg total RNA without
any blocking agents such as BSA,
but the competitor has higher background signal in spite
of being treated with BSA.
Figure 1. Fluorescence images after hybridization
with cDNAs reverse-transcripted from total RNA. NSB
and competitor slides were tested.
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